Binding Assay:Article Title:
Article Snippet: Arg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494
Article Title:
Article Snippet: Supplementary Information vPIF-1 is an insulin-like anti-ferroptotic viral peptide Supplementary Material and Methods Reagents *the structure of MIA-602 is PhAC-Ada-Tyr-D-Arg-Asp-Ala-Ile-5FPhe-Thr-Ala-HarTyr(Me)-His-Orn-Val-Leu-Abu-Gln-Leu-Ser-Ala-His-Orn-Leu-Leu-Gln-Asp-Ile-Ale-DArg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494 Ferroptocide Provided by Paul J. Hergenrother n/a TNFα human BioLegend #570108 Birinapant Chemietek #CT-BIRI zVAD fmk BD Biosciences 550377 Nec-1s (7-O-Cl-Nec-1) Merck Millipore #5.04297.0001 Mitotane Sigma Aldrich #SML1885 MIA-602 Provided by A. V. Schally n/a* ITS+1 Sigma Aldrich #I2521 LCDV-1 Peptide Phoenix Pharmaceuticals, Inc. #036-61 Des-C-Peptide LCDV-1 Phoenix Pharmaceuticals, Inc. #036-58 C-peptide (human) Phoenix Pharmaceuticals, Inc. #035-01 insulin solution (human) Sigma Aldrich #I9278 Annexin V, Alexa FluorTM 647 conjugate ThermoFisher Scientific #A23204 7-AAD BD Biosciences #559925 Annexin-V-FITC BD Biosciences #556420 Annexin-V binding buffer BD Biosciences #556454 BODIPY 581/591 ThermoFisher Scientific #D3861 Bradford assay Thermo Fisher Scientific #23225 ECLTM Prime Western Blotting System Thermo Fisher Scientific #GERPN2232 Kits CyQUANT® Cell Proliferation Assay Kit Invitrogen #C7026 Cell lines and cell culture Human HT1080 and mouse NIH-3T3 cell lines were purchased from the American Type Culture Collection, while the rest were kindly provided by collaborators.
Bradford Assay:Article Title:
Article Snippet: Arg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494
Article Title:
Article Snippet: Supplementary Information vPIF-1 is an insulin-like anti-ferroptotic viral peptide Supplementary Material and Methods Reagents *the structure of MIA-602 is PhAC-Ada-Tyr-D-Arg-Asp-Ala-Ile-5FPhe-Thr-Ala-HarTyr(Me)-His-Orn-Val-Leu-Abu-Gln-Leu-Ser-Ala-His-Orn-Leu-Leu-Gln-Asp-Ile-Ale-DArg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494 Ferroptocide Provided by Paul J. Hergenrother n/a TNFα human BioLegend #570108 Birinapant Chemietek #CT-BIRI zVAD fmk BD Biosciences 550377 Nec-1s (7-O-Cl-Nec-1) Merck Millipore #5.04297.0001 Mitotane Sigma Aldrich #SML1885 MIA-602 Provided by A. V. Schally n/a* ITS+1 Sigma Aldrich #I2521 LCDV-1 Peptide Phoenix Pharmaceuticals, Inc. #036-61 Des-C-Peptide LCDV-1 Phoenix Pharmaceuticals, Inc. #036-58 C-peptide (human) Phoenix Pharmaceuticals, Inc. #035-01 insulin solution (human) Sigma Aldrich #I9278 Annexin V, Alexa FluorTM 647 conjugate ThermoFisher Scientific #A23204 7-AAD BD Biosciences #559925 Annexin-V-FITC BD Biosciences #556420 Annexin-V binding buffer BD Biosciences #556454 BODIPY 581/591 ThermoFisher Scientific #D3861 Bradford assay Thermo Fisher Scientific #23225 ECLTM Prime Western Blotting System Thermo Fisher Scientific #GERPN2232 Kits CyQUANT® Cell Proliferation Assay Kit Invitrogen #C7026 Cell lines and cell culture Human HT1080 and mouse NIH-3T3 cell lines were purchased from the American Type Culture Collection, while the rest were kindly provided by collaborators.
Western Blot:Article Title:
Article Snippet: Arg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494
Article Title:
Article Snippet: Supplementary Information vPIF-1 is an insulin-like anti-ferroptotic viral peptide Supplementary Material and Methods Reagents *the structure of MIA-602 is PhAC-Ada-Tyr-D-Arg-Asp-Ala-Ile-5FPhe-Thr-Ala-HarTyr(Me)-His-Orn-Val-Leu-Abu-Gln-Leu-Ser-Ala-His-Orn-Leu-Leu-Gln-Asp-Ile-Ale-DArg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494 Ferroptocide Provided by Paul J. Hergenrother n/a TNFα human BioLegend #570108 Birinapant Chemietek #CT-BIRI zVAD fmk BD Biosciences 550377 Nec-1s (7-O-Cl-Nec-1) Merck Millipore #5.04297.0001 Mitotane Sigma Aldrich #SML1885 MIA-602 Provided by A. V. Schally n/a* ITS+1 Sigma Aldrich #I2521 LCDV-1 Peptide Phoenix Pharmaceuticals, Inc. #036-61 Des-C-Peptide LCDV-1 Phoenix Pharmaceuticals, Inc. #036-58 C-peptide (human) Phoenix Pharmaceuticals, Inc. #035-01 insulin solution (human) Sigma Aldrich #I9278 Annexin V, Alexa FluorTM 647 conjugate ThermoFisher Scientific #A23204 7-AAD BD Biosciences #559925 Annexin-V-FITC BD Biosciences #556420 Annexin-V binding buffer BD Biosciences #556454 BODIPY 581/591 ThermoFisher Scientific #D3861 Bradford assay Thermo Fisher Scientific #23225 ECLTM Prime Western Blotting System Thermo Fisher Scientific #GERPN2232 Kits CyQUANT® Cell Proliferation Assay Kit Invitrogen #C7026 Cell lines and cell culture Human HT1080 and mouse NIH-3T3 cell lines were purchased from the American Type Culture Collection, while the rest were kindly provided by collaborators.
CyQUANT Assay:Article Title:
Article Snippet: Arg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494
Article Title:
Article Snippet: Supplementary Information vPIF-1 is an insulin-like anti-ferroptotic viral peptide Supplementary Material and Methods Reagents *the structure of MIA-602 is PhAC-Ada-Tyr-D-Arg-Asp-Ala-Ile-5FPhe-Thr-Ala-HarTyr(Me)-His-Orn-Val-Leu-Abu-Gln-Leu-Ser-Ala-His-Orn-Leu-Leu-Gln-Asp-Ile-Ale-DArg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494 Ferroptocide Provided by Paul J. Hergenrother n/a TNFα human BioLegend #570108 Birinapant Chemietek #CT-BIRI zVAD fmk BD Biosciences 550377 Nec-1s (7-O-Cl-Nec-1) Merck Millipore #5.04297.0001 Mitotane Sigma Aldrich #SML1885 MIA-602 Provided by A. V. Schally n/a* ITS+1 Sigma Aldrich #I2521 LCDV-1 Peptide Phoenix Pharmaceuticals, Inc. #036-61 Des-C-Peptide LCDV-1 Phoenix Pharmaceuticals, Inc. #036-58 C-peptide (human) Phoenix Pharmaceuticals, Inc. #035-01 insulin solution (human) Sigma Aldrich #I9278 Annexin V, Alexa FluorTM 647 conjugate ThermoFisher Scientific #A23204 7-AAD BD Biosciences #559925 Annexin-V-FITC BD Biosciences #556420 Annexin-V binding buffer BD Biosciences #556454 BODIPY 581/591 ThermoFisher Scientific #D3861 Bradford assay Thermo Fisher Scientific #23225 ECLTM Prime Western Blotting System Thermo Fisher Scientific #GERPN2232 Kits CyQUANT® Cell Proliferation Assay Kit Invitrogen #C7026 Cell lines and cell culture Human HT1080 and mouse NIH-3T3 cell lines were purchased from the American Type Culture Collection, while the rest were kindly provided by collaborators.
Proliferation Assay:Article Title:
Article Snippet: Arg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494
Article Title:
Article Snippet: Supplementary Information vPIF-1 is an insulin-like anti-ferroptotic viral peptide Supplementary Material and Methods Reagents *the structure of MIA-602 is PhAC-Ada-Tyr-D-Arg-Asp-Ala-Ile-5FPhe-Thr-Ala-HarTyr(Me)-His-Orn-Val-Leu-Abu-Gln-Leu-Ser-Ala-His-Orn-Leu-Leu-Gln-Asp-Ile-Ale-DArg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494 Ferroptocide Provided by Paul J. Hergenrother n/a TNFα human BioLegend #570108 Birinapant Chemietek #CT-BIRI zVAD fmk BD Biosciences 550377 Nec-1s (7-O-Cl-Nec-1) Merck Millipore #5.04297.0001 Mitotane Sigma Aldrich #SML1885 MIA-602 Provided by A. V. Schally n/a* ITS+1 Sigma Aldrich #I2521 LCDV-1 Peptide Phoenix Pharmaceuticals, Inc. #036-61 Des-C-Peptide LCDV-1 Phoenix Pharmaceuticals, Inc. #036-58 C-peptide (human) Phoenix Pharmaceuticals, Inc. #035-01 insulin solution (human) Sigma Aldrich #I9278 Annexin V, Alexa FluorTM 647 conjugate ThermoFisher Scientific #A23204 7-AAD BD Biosciences #559925 Annexin-V-FITC BD Biosciences #556420 Annexin-V binding buffer BD Biosciences #556454 BODIPY 581/591 ThermoFisher Scientific #D3861 Bradford assay Thermo Fisher Scientific #23225 ECLTM Prime Western Blotting System Thermo Fisher Scientific #GERPN2232 Kits CyQUANT® Cell Proliferation Assay Kit Invitrogen #C7026 Cell lines and cell culture Human HT1080 and mouse NIH-3T3 cell lines were purchased from the American Type Culture Collection, while the rest were kindly provided by collaborators.
Cell Culture:Article Title:
Article Snippet: Arg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494
Article Title:
Article Snippet: Supplementary Information vPIF-1 is an insulin-like anti-ferroptotic viral peptide Supplementary Material and Methods Reagents *the structure of MIA-602 is PhAC-Ada-Tyr-D-Arg-Asp-Ala-Ile-5FPhe-Thr-Ala-HarTyr(Me)-His-Orn-Val-Leu-Abu-Gln-Leu-Ser-Ala-His-Orn-Leu-Leu-Gln-Asp-Ile-Ale-DArg-Ile-D-Arg-Har-NH2 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αFASL human, CH11 Merck Millipore #05-201 Insulin receptor β (IRβ) Cell Signaling #23413 type I insulin-like growth factor receptor beta (IGF-IRβ) Cell Signaling #9750 Akt Cell Signaling #9272 p-Akt Cell Signaling #9271 β-actin Cell Signaling Cell Signaling #3700S Anti-mouse IgG; HRP-linked antibody Cell Signaling #7076S Anti-rabbit IgG; HRP-linked antibody Cell Signaling #7074S Compounds & chemicals Erastin (type I FIN) Sigma Aldrich #E7781 RSL3 (type II FIN) Selleck Chemicals #S8155 FIN56 (type III FIN) Sigma Aldrich #SML1740 FINO2 (type IV FIN) Cayman Chemical #25096 Ferrostatin-1 (Fer-1) Merck Millipore #341494 Ferroptocide Provided by Paul J. Hergenrother n/a TNFα human BioLegend #570108 Birinapant Chemietek #CT-BIRI zVAD fmk BD Biosciences 550377 Nec-1s (7-O-Cl-Nec-1) Merck Millipore #5.04297.0001 Mitotane Sigma Aldrich #SML1885 MIA-602 Provided by A. V. Schally n/a* ITS+1 Sigma Aldrich #I2521 LCDV-1 Peptide Phoenix Pharmaceuticals, Inc. #036-61 Des-C-Peptide LCDV-1 Phoenix Pharmaceuticals, Inc. #036-58 C-peptide (human) Phoenix Pharmaceuticals, Inc. #035-01 insulin solution (human) Sigma Aldrich #I9278 Annexin V, Alexa FluorTM 647 conjugate ThermoFisher Scientific #A23204 7-AAD BD Biosciences #559925 Annexin-V-FITC BD Biosciences #556420 Annexin-V binding buffer BD Biosciences #556454 BODIPY 581/591 ThermoFisher Scientific #D3861 Bradford assay Thermo Fisher Scientific #23225 ECLTM Prime Western Blotting System Thermo Fisher Scientific #GERPN2232 Kits CyQUANT® Cell Proliferation Assay Kit Invitrogen #C7026 Cell lines and cell culture Human HT1080 and mouse NIH-3T3 cell lines were purchased from the American Type Culture Collection, while the rest were kindly provided by collaborators.
| 
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 